4 elements ii igg1/31/2024 These chains cover the hydrophobic faces that would normally lead to domain pairing. As displayed in the image above the Fc region of an IgG consists of two paired CH3 domains and, in contrast, two CH2 domains that are separated and do not interact but have two oligosaccharide chains interposed between them. PDB accession number of the mouse IgG1 is 1IGY.Īntibodies are glycosylated proteins, with the position and extent of glycosylation varying between isotypes. On the left is a ribbon representation showing the secondary structure elements and on the right hand side is a space-filled model of the same molecule. The heavy chain is shown in blue, light chain in green and glycosylation in orange. It is the CDRs that provide a specific antigen recognition site on the surface of the antibody and the hypervariability of these regions enables antibodies to recognize an almost unlimited number of antigens (3).įigure. Each variable domain contains three hypervariable loops, known as complementarity determining regions (CDRs), evenly distributed between four less variable framework (FR) regions. The Fab domains consist of two variable and two constant domains, with the two variable domains making up the variable fragment (Fv), which provides the antigen specificity of the antibody (2) with the constant domains acting as a structural framework. The chains fold into repeated immunoglobulin folds consisting of anti-parallel β-sheets (1), which form either constant or variable domains. The enlargement of the variable domain shows a ribbon representation of the β-sheet framework and CDR loops.Īntibodies all have the same basic structure consisting of two heavy and two light chains forming two Fab arms containing identical domains at either end attached by a flexible hinge region to the stem of the antibody, the Fc domain, giving the classical ‘Y’ shape. Schematic representation of an IgG.Īn antibody consists of two heavy chains (blue) and two light chains (green) folded into constant and variable domains. FleXpress™ High-Throughput Antibody Productionįigure.Immunoglobulin Fc domains (Ig Fc domains).Anti-Non-Human Primate (NHP) Immunoglobulin Antibodies.VivopureX™ Antibodies for In Vivo Research.Recombinant Antibodies from Human Patients.
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